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Gestational Diabetes Screening: One-Step vs Two-Step and What to Expect

GDM screening at 24-28 weeks, one-step vs two-step glucose tolerance testing, who needs early screening, and what the numbers mean.

Published January 14, 2026 · Updated April 30, 2026 · Medically reviewed by HerCalc Editorial Team

Gestational diabetes (GDM) is glucose intolerance first identified in pregnancy. It affects roughly 6 to 9 percent of pregnancies in the United States, with rates rising along with background obesity and average maternal age. Diagnosed and managed, most pregnancies with GDM have outcomes very close to non-diabetic pregnancies. Undiagnosed, GDM raises risks of macrosomia, shoulder dystocia, neonatal hypoglycemia, pre-eclampsia, and cesarean delivery.

Screening is universal in the US. Below is what to expect, what the two main testing approaches look like, and what the diagnostic numbers actually mean.

When screening happens

ACOG Practice Bulletin 190 (2018, reaffirmed) recommends:

Risk factors that trigger early screening:

For more on PCOS as a risk factor, see PCOS and fertility and our post on insulin resistance.

One-step vs two-step: the long debate

There are two main approaches in current use, and US practice varies.

Two-step (Carpenter-Coustan), the long-standing US standard

  1. 50-gram glucose challenge test (GCT). Non-fasting. Drink 50g glucose, blood draw at 1 hour. Threshold typically 140 mg/dL (some institutions use 130 or 135). About 15 to 20 percent of people screen positive.
  2. 100-gram 3-hour oral glucose tolerance test (OGTT). Fasting. Blood draws fasting, 1, 2, and 3 hours after the 100g drink. GDM is diagnosed if 2 or more of the 4 values exceed thresholds (typically 95, 180, 155, 140 mg/dL).

Roughly 2 to 3 percent of all screened people end up with a GDM diagnosis using two-step.

One-step (IADPSG / WHO), the international approach

A single 75-gram 2-hour OGTT, fasting. Diagnosis if any one of:

One-step diagnoses about twice as many people as two-step (roughly 6 to 9 percent).

Which is better?

Hillier et al. (NEJM 2021) directly compared the two strategies in a randomized trial (ScreenR2GDM) of over 23,000 pregnant people. The result: one-step doubled the GDM diagnosis rate but did not significantly improve perinatal outcomes (large-for-gestational-age, primary cesarean, hypertensive disorders, neonatal hypoglycemia, perinatal composite).

This supported ACOG’s continued recommendation that either approach is acceptable, with two-step remaining the most common US standard. The trial did not settle the broader question — there is ongoing debate about whether the one-step approach catches mild cases that benefit from earlier intervention even if trial-level outcomes are similar.

What you experience depends on your hospital’s chosen pathway.

What the numbers mean

If your 1-hour screen comes back at 145, the next step is the 3-hour test, not a diagnosis. About 1 in 4 people who screen positive on the 1-hour go on to fail the 3-hour. The remaining 3 in 4 have a normal 3-hour and no GDM.

If your 1-hour is dramatically elevated (often above 200), some institutions skip directly to diagnosis or proceed straight to A1C and management — confirm with your provider.

A normal screen reduces but does not eliminate GDM probability. If you develop concerning findings later (excessive fetal growth, polyhydramnios), retesting later in pregnancy is reasonable.

What treatment looks like

Mild GDM is managed with:

If diet and movement do not bring numbers into target after 1 to 2 weeks, medication is added. Insulin is the first-line in pregnancy. Metformin is sometimes used and is considered acceptable in current ACOG guidance, though insulin remains the gold standard. Glyburide use has fallen out of favor.

What GDM means for delivery

After delivery

Where this fits in your timeline

GDM screening lands in the same window as anatomy ultrasound and the start of third-trimester surveillance. The Pregnancy Week Calculator helps you track when 24 to 28 weeks lands relative to your due date.

Questions worth asking

The bottom line

GDM screening is universal at 24 to 28 weeks, with earlier testing for higher-risk people. The 50-gram 1-hour screen plus the 3-hour confirmatory test (two-step) remains the US standard, but the 75-gram one-step is also accepted. Hillier et al. NEJM 2021 found similar outcomes between strategies despite different diagnosis rates. Most GDM is managed with diet and movement; some needs insulin. Postpartum follow-up testing matters for long-term health and is too often skipped. Knowing what the test is and what the numbers mean keeps the diagnosis from feeling like a black box.

Frequently asked questions

Do I have to drink the orange glucose drink? +

The standard glucose load is a measured 50, 75, or 100-gram dose of glucose in flavored solution. Some clinics offer alternatives like jellybean equivalents, though calibration is less standardized. The drink itself is not dangerous in pregnancy — the volume of glucose is the same as a large soda or two pieces of cake, just delivered faster.

Can I prepare for the test? +

For the 1-hour 50g screen, no fasting is required and you can eat normally that day. For the 3-hour 100g test or the 2-hour 75g test, fast 8 to 12 hours overnight. Do not crash-diet beforehand, as severe carbohydrate restriction the day before can produce a false positive. Eat normally for 3 days prior, then fast as instructed.

I had GDM in a prior pregnancy. Do I still need screening? +

Yes, and earlier. ACOG recommends screening at the first prenatal visit (or by 12-14 weeks) for anyone with prior GDM, BMI 30 or higher, family history of type 2 diabetes, prior macrosomia, PCOS, or other risk factors. If early screening is normal, repeat the standard 24 to 28-week test.

HerCalc content is for educational use only and does not replace professional medical advice. If you are concerned about a symptom or making a treatment decision, please contact a qualified healthcare provider.